Transgenic Mice Carrying the Human Poliovirus Receptor: New Animal Model for Study of Poliovirus Neurovirulence
نویسنده
چکیده
Department of Microbiology' and Department of Laboratory Animal Science,5 Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, Tokyo 113, Department of Pathology, National Institute of Health, Toyama, Shinjuku-ku, Tokyo 162,3 Japan Poliomyelitis Research Institute, Higashimurayama, Tokyo 189,2 Central Institlite for Experimental Animals, Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216,4 and Department of Microbiology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108," Japan7
منابع مشابه
Transgenic mice carrying the human poliovirus receptor: new animal models for study of poliovirus neurovirulence.
Recombinant viruses between the virulent Mahoney and attenuated Sabin 1 strains of poliovirus type 1 were subjected to neurovirulence tests using a transgenic (Tg) mouse line, ICR-PVRTg1, that carried the human poliovirus receptor gene. The Tg mice were inoculated intracerebrally with these recombinant viruses and observed for clinical signs, histopathological lesions, and viral antigens as par...
متن کاملMolecular aspects of poliovirus pathogenesis
The development of a transgenic mouse model carrying the human poliovirus receptor has made it possible to investigate the molecular mechanisms of the viral dissemination process in a whole organism. Studies on this have provided an insight into the mechanisms for viral permeation through the blood-brain barrier and retrograde axonal transport of the virus. In addition, strain-specific neurovir...
متن کاملTransgenic mice as an alternative to monkeys for neurovirulence testing of live oral poliovirus vaccine: validation by a WHO collaborative study.
OBJECTIVE Extensive WHO collaborative studies were performed to evaluate the suitability of transgenic mice susceptible to poliovirus (TgPVR mice, strain 21, bred and provided by the Central Institute for Experimental Animals, Japan) as an alternative to monkeys in the neurovirulence test (NVT) of oral poliovirus vaccine (OPV). METHODS Nine laboratories participated in the collaborative study...
متن کاملMucosal and systemic immunity against poliovirus in mice transgenic for the poliovirus receptor: the poliovirus receptor is necessary for a virus-specific mucosal IgA response.
In view of the planned eradication of poliovirus, the suitability of transgenic mice bearing the human receptor for poliovirus (PVRtg mice) as a nonprimate animal model to study mucosal immunity against poliovirus was investigated. After intraperitoneal (ip) priming followed by ip or oral booster with live poliovirus, PVRtg mice had detectable IgA and IgG responses. The IgA response was restric...
متن کاملOncolytic treatment and cure of neuroblastoma by a novel attenuated poliovirus in a novel poliovirus-susceptible animal model.
Neuroblastoma is one of the most common solid tumors in children. Treatment is of limited utility for high-risk neuroblastoma and prognosis is poor. Resistance of neuroblastoma to conventional therapies has prompted us to search for a novel therapeutic approach based on genetically modified polioviruses. Poliovirus targets motor neurons leading to irreversible paralysis. Neurovirulence can be a...
متن کامل